10% BUFFERED FORMALIN

What is 10% Buffered Formalin?

10% buffered formalin is a biological fixative used in scientific research as a preservative chemical composition. This solution consists of 10% formaldehyde gas dissolved in water, buffered with sodium phosphate or another buffer to maintain a stable pH level. It is widely used in scientific laboratories to preserve tissue samples and maintain their structural integrity for further analysis.

Preservative Chemical Composition

The preservative chemical composition of 10% buffered formalin includes:

• Formaldehyde gas: 10%
• Sodium phosphate buffer: variable concentration
• Water: remaining volume

Applications in Scientific Research

10% buffered formalin has numerous applications in scientific research, including:

• Histology: Fixing tissue samples for microscopic examination
• Pathology: Preserving human and animal tissues for diagnostic purposes
• Immunohistochemistry: Stabilizing proteins in tissue samples for antibody binding

How to Use 10% Buffered Formalin

Using 10% buffered formalin requires proper storage, handling, and safety precautions.

Proper Storage & Handling

Store 10% buffered formalin in a cool, dry place away from direct sunlight and heat sources. Keep the container tightly sealed to prevent evaporation and contamination. When handling the solution, wear appropriate personal protective equipment (PPE) such as gloves, goggles, and lab coats.

Safety Precautions

Formalin is a hazardous substance and must be handled with care. Follow these safety precautions:

• Avoid skin contact and inhalation of fumes
• Work in a well-ventilated area or under a fume hood
• Dispose of used formalin solution according to local regulations

Formalin Solution: An Overview

Formalin solution has a rich history and development, starting from its discovery in the late 19th century. It has since become an essential component in scientific laboratories worldwide.

History & Development

Formalin was first synthesized by German chemist August von Hofmann in 1867. It gained popularity in scientific research during the early 20th century due to its effectiveness as a biological fixative.

Other Common Formalin Concentrations

In addition to 10% buffered formalin, other common concentrations include:

• 4% neutral buffered formalin (NBF)
• 15% formalin solution

Formalin Solution in Scientific Laboratory Supplies

When purchasing and maintaining formalin solution, it is crucial to adhere to best practices.

Best Practices for Purchasing & Maintenance

Follow these guidelines when buying and maintaining formalin solution:

• Purchase from reputable suppliers
• Store in a secure location away from incompatible substances
• Regularly inspect containers for signs of damage or leakage

Compatibility with Other Laboratory Equipment

Formalin solution may interact with certain laboratory equipment, such as metal containers or glassware. To avoid contamination or degradation, use compatible materials like polyethylene or polypropylene containers and avoid prolonged contact with metal surfaces.

In summary, 10% buffered formalin is a vital biological fixative in scientific research, with applications ranging from histology to immunohistochemistry. Proper storage, handling, and safety precautions are essential when working with this hazardous substance. By following best practices for purchasing and maintenance, researchers can ensure the continued effectiveness of formalin solution in their laboratories.